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teachings:tuto_16s_microbial_analysis [2020/09/08 16:15] olivierteachings:tuto_16s_microbial_analysis [2020/09/11 09:44] olivier
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   * To know what a fasta or fastq (fastaq = fq) file is   * To know what a fasta or fastq (fastaq = fq) file is
- <hidden Click here for fasta format description>  + <hidden Click here for the fasta format description>  
-//Data in text format used to store nucleic acid sequences (such as DNA sequences) or protein sequences; may contain multiple sequences. FASTA files often start with a header line that may contain comments or other information. The rest of the file contains sequence data. Each sequence starts with a ">" symbol followed by the name of the sequence. The rest of the line describes the sequence and the remaining lines contain the sequence itself.// \\Example : \\ \\ <nowiki>+//Data in text format used to store nucleic acid sequences (such as DNA sequences) or protein sequences; may contain multiple sequences. FASTA files often start with a header line that may contain comments or other information. The rest of the file contains sequence data. Each sequence starts with a ">" symbol followed by the name of the sequence. The rest of the line describes the sequence and the remaining lines contain the sequence itself.// \\ \\ Example of a fasta file including 2 sequences:\\ \\ 
 +<nowiki>
 >human_T1 (UCSC April 2002 chr7:115977709: >human_T1 (UCSC April 2002 chr7:115977709:
 TTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGCTAAGGGCAGCC TTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGCTAAGGGCAGCC
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 ATCTCTCGGCAGAAACCCTACAGGCCAGAAGAGAGTGGGGGCCAATATT ATCTCTCGGCAGAAACCCTACAGGCCAGAAGAGAGTGGGGGCCAATATT
 CATATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTCATATCCAGCCAA CATATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTCATATCCAGCCAA
-</nowiki> 
- 
-<nowiki> 
 >human_T2 (UCSC April 2002 chr5:11977710: >human_T2 (UCSC April 2002 chr5:11977710:
 ATACGACYCTTATTGTTAGTATATAATTTATATGAAAACMAAAAATTATG ATACGACYCTTATTGTTAGTATATAATTTATATGAAAACMAAAAATTATG
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 </hidden> </hidden>
 \\ \\
- +  * an access to a Galaxy website serverincluding usual tools for metagenomic analysis
-  * an access to a Galaxy website server including usual tools for metagenomic analysis+
  
 See what is the Galaxy project: [[https://en.wikipedia.org/wiki/Galaxy_%28computational_biology%29|Wikipedia]] or [[https://usegalaxy.org/|Official website]] See what is the Galaxy project: [[https://en.wikipedia.org/wiki/Galaxy_%28computational_biology%29|Wikipedia]] or [[https://usegalaxy.org/|Official website]]
  
 {{ :teachings:galaxy.png?400 |}} {{ :teachings:galaxy.png?400 |}}
-//Main display of Galaxy (using a browser)//+//Example of the main display page of Galaxy (using a browser)//
  
 => __3 possibilities to get access to a Galaxy server__: => __3 possibilities to get access to a Galaxy server__:
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 __pro__: no installation required, usable directly \\ __pro__: no installation required, usable directly \\
-__cons__: slow (or super slow) when running big computations+__cons__: some limitations and slow (or super slow) when running big computations
  
  **2)** Use our local Galaxy server (IRCAN institute, in Pasteur tower)  **2)** Use our local Galaxy server (IRCAN institute, in Pasteur tower)
  
- http://wolverine.unice.fr:8080/+ http://galaxy-metag.ircan.org/ (alternate link: http://wolverine.unice.fr:8080/)
  
 __pro__: no installation required, usable directly\\ __pro__: no installation required, usable directly\\
-__cons__: should to be faster than previous servers, but depending the numbers of students connected at the same time !+__cons__: should to be faster than previous servers, but depending the number of students connected at the same time !
  
  **3)** Install Galaxy on your own computer  **3)** Install Galaxy on your own computer
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   * Create a personal account into Galaxy (top-right menu)    * Create a personal account into Galaxy (top-right menu) 
  
-  * Start this tutorial : [[https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop-short/tutorial.html|here]]+  * Start this tutorial : [[http://www.bioinfomed.fr/__Teachings/tuto_Galaxy_metaG/index.html|Here]] //([[https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop-short/tutorial.html|alternative link]])//
  
teachings/tuto_16s_microbial_analysis.txt · Last modified: 2022/10/13 14:12 by olivier