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16S Microbial Analysis (with Galaxy)

This tutorial required:

  • a computer (under Linux, Mac, Windows) with an internet connection
  • an access to a Galaxy website server (many are available freely)

    ⇒ See what is the Galaxy project: Wikipedia or Official website

  • To know what a fasta or fastq (fastaq = fq) file is :

Data in text format used to store nucleic acid sequences (such as DNA sequences) or protein sequences; may contain multiple sequences. FASTA files often start with a header line that may contain comments or other information. The rest of the file contains sequence data. Each sequence starts with a “>” symbol followed by the name of the sequence. The rest of the line describes the sequence and the remaining lines contain the sequence itself. Example :

>human_T1 (UCSC April 2002 chr7:115977709:) TTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGCTAAGGGCAGCC AGAGAGAGGTCAGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCG ATCTCTCGGCAGAAACCCTACAGGCCAGAAGAGAGTGGGGGCCAATATT CATATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTCATATCCAGCCAA

>human_T2 (UCSC April 2002 chr5:11977710:) ATACGACYCTTATTGTTAGTATATAATTTATATGAAAACMAAAAATTATG GCGGTATTTTAAGCTTTTCAGAGGAATTTGCTCTTTAATGGATAAAAC CTAAATCTTACTAGAATTAGTAAAGCAGTTTGTATACCACT

  • Start this tutrorial : here
teachings/tuto_16s_microbial_analysis.1581092484.txt.gz · Last modified: 2020/02/07 17:21 by olivier