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16S Microbial Analysis (with Galaxy)

This tutorial required:

  • a computer (under Linux, Mac, Windows) with an internet connection
  • To know what a fasta or fastq (fastaq = fq) file is

Click here for fasta format description

Click here for fasta format description

Data in text format used to store nucleic acid sequences (such as DNA sequences) or protein sequences; may contain multiple sequences. FASTA files often start with a header line that may contain comments or other information. The rest of the file contains sequence data. Each sequence starts with a “>” symbol followed by the name of the sequence. The rest of the line describes the sequence and the remaining lines contain the sequence itself. \\Example :

>human_T1 (UCSC April 2002 chr7:115977709:) TTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGCTAAGGGCAGCC AGAGAGAGGTCAGGTTACCCACAAAGGGAAGCCCATCAGACTAACAGCG ATCTCTCGGCAGAAACCCTACAGGCCAGAAGAGAGTGGGGGCCAATATT CATATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTCATATCCAGCCAA

>human_T2 (UCSC April 2002 chr5:11977710:) ATACGACYCTTATTGTTAGTATATAATTTATATGAAAACMAAAAATTATG GCGGTATTTTAAGCTTTTCAGAGGAATTTGCTCTTTAATGGATAAAAC CTAAATCTTACTAGAATTAGTAAAGCAGTTTGTATACCACT


  • an access to a Galaxy website server including usual tools for metagenomic analysis

- See what is the Galaxy project: Wikipedia or Official website

⇒ 3 solutions:

* Use a freely distant server 

many servers are available, here a list of servers with metaG tools:
 freely): \\ \\



https://usegalaxy.org.au/login
https://usegalaxy.eu/login
https://nanopore.usegalaxy.eu/login
https://ecology.usegalaxy.eu/login

  • Create an account on of these Galaxy server : * google it for others servers !
  • ⇒ Start this tutorial : here
teachings/tuto_16s_microbial_analysis.1599573062.txt.gz · Last modified: 2020/09/08 15:51 by olivier

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